The miR-146a SNP Rs2910164 and miR-155 SNP rs767649 are risk factors for non-small cell lung cancer in the Iranian population
Author(s)
Type
Journal Article
Abstract
Background. Lung cancer is one of the leading causes of death worldwide. MicroRNAs (miRNAs) are small noncoding RNAs that
regulate gene expression and may act as both tumor suppressors and as oncogenes. *e presence of single nucleotide polymorphisms (SNPs) inside the miRNA genomic region could affect target miRNA maturation, expression, and binding to its target
mRNA and contribute to cancer development. Previous studies on the SNPs Rs2910164 in miR-146a and Rs767649 in miR-155
showed association with non-small cell lung cancer (NSCLC) development. *us, the aim of this study was to detect any
correlation between those SNPs in Iranian NSCLC patients. Methods. In a small cohort study, 165 NSCLC patients and 147
noncancer controls were enrolled between Apr 2015 and Sep 2019 at the Masih Daneshvari Hospital, Tehran, Iran. Allele
frequencies from the genomic DNA of blood cells were studied using PCR-RFLP and their association with the risk of lung cancer
was evaluated. Results. *e rs2910164C allele (OR � 1.56, 95% CI � 1.10–2.21, p � 0.012) and CC genotype (OR � 2.93, 95%
CI � 1.07–7.9, p � 0.034, respectively) were associated with a significantly increased risk for lung cancer compared to that for the
GG genotype. When patients were stratified according to smoking exposure, no association with rs2910164 variants was found.
*e AT genotype (OR � 0.57, 95% CI � 0.33–0.99, p � 0.048) and the A allele frequency (OR � 0.58, 95% CI � 0.35–0.98,
p � 0.043) in rs767649 were lower in NSCLC patients in comparison with the control group. In addition, the rs767649 AT
genotype frequency in smoking controls was higher than in smoking NSCLC patients (OR � 0.44, 95% CI � 0.21–0.90, p � 0.024).
No association was found between rs2910164 and rs767649 variants and stage or type of NSCLC. Conclusion. Our finding suggests
that miR-146a rs2910164 and miR-155 rs767649 polymorphisms may be considered as genetic risk factors for the susceptibility to
NSCLC in the Iranian population. However, a larger multicenter study across Iran is needed to confirm these findings.
regulate gene expression and may act as both tumor suppressors and as oncogenes. *e presence of single nucleotide polymorphisms (SNPs) inside the miRNA genomic region could affect target miRNA maturation, expression, and binding to its target
mRNA and contribute to cancer development. Previous studies on the SNPs Rs2910164 in miR-146a and Rs767649 in miR-155
showed association with non-small cell lung cancer (NSCLC) development. *us, the aim of this study was to detect any
correlation between those SNPs in Iranian NSCLC patients. Methods. In a small cohort study, 165 NSCLC patients and 147
noncancer controls were enrolled between Apr 2015 and Sep 2019 at the Masih Daneshvari Hospital, Tehran, Iran. Allele
frequencies from the genomic DNA of blood cells were studied using PCR-RFLP and their association with the risk of lung cancer
was evaluated. Results. *e rs2910164C allele (OR � 1.56, 95% CI � 1.10–2.21, p � 0.012) and CC genotype (OR � 2.93, 95%
CI � 1.07–7.9, p � 0.034, respectively) were associated with a significantly increased risk for lung cancer compared to that for the
GG genotype. When patients were stratified according to smoking exposure, no association with rs2910164 variants was found.
*e AT genotype (OR � 0.57, 95% CI � 0.33–0.99, p � 0.048) and the A allele frequency (OR � 0.58, 95% CI � 0.35–0.98,
p � 0.043) in rs767649 were lower in NSCLC patients in comparison with the control group. In addition, the rs767649 AT
genotype frequency in smoking controls was higher than in smoking NSCLC patients (OR � 0.44, 95% CI � 0.21–0.90, p � 0.024).
No association was found between rs2910164 and rs767649 variants and stage or type of NSCLC. Conclusion. Our finding suggests
that miR-146a rs2910164 and miR-155 rs767649 polymorphisms may be considered as genetic risk factors for the susceptibility to
NSCLC in the Iranian population. However, a larger multicenter study across Iran is needed to confirm these findings.
Date Issued
2020-11-21
Date Acceptance
2020-10-30
Citation
Canadian Respiratory Journal, 2020, 2020, pp.1-8
ISSN
1198-2241
Publisher
Hindawi
Start Page
1
End Page
8
Journal / Book Title
Canadian Respiratory Journal
Volume
2020
Copyright Statement
© 2020 Neda K. Dezfuli et al. *is is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.
License URL
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000595976000001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Respiratory System
POLYMORPHISM
MIR-196A2
CONTRIBUTES
ASSOCIATION
Publication Status
Published
Article Number
ARTN 8179415
Date Publish Online
2020-11-21