Label-free single-molecule imaging of single protein pores
File(s)
Author(s)
Parperis, Christopher
Type
Thesis
Abstract
Interferometric scattering microscopy is a label-free all optical technique which overcomes many of the inherent limitations of fluorescence-based approaches. Efficient subtraction of the large reference signal and its magnitude relative to the scattering of the object is key to high-speed high-sensitivity imaging in complex biological samples. In this research, methods for improving the SNR are explored with a view to developing new experimental procedures for studying single-molecule interactions on lipid membranes, including adaptive Fourier filtering and refractive index matching for enhanced interferometric signal contrast. In addition to this preliminary work, single proteins are visualised interacting on artificial bilayer membranes by iSCAT and observed to assemble into multimeric pore structures.
Version
Open Access
Date Issued
2022-04-30
Date Awarded
01/03/2023
License URL
Advisor
Ces, Oscar
Wallace, Mark
Sponsor
Engineering and Physical Sciences Research Council
Oxford Nanopore Technologies (Firm)
Publisher Department
Chemistry
Publisher Institution
Imperial College London
Qualification Level
Doctoral
Qualification Name
Doctor of Philosophy (PhD)