N-methyl deuterated rhodamines for protein labelling in sensitive fluorescence microscopy
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Rhodamine fluorophores are setting benchmarks in fluorescence microscopy. Herein, we report the deuterium (d12) congeners of tetramethyl(silicon)rhodamine, obtained by isotopic labelling of the four methyl groups, show improved photophysical parameters (i.e. brightness, lifetimes) and reduced chemical bleaching. We explore this finding for SNAP- and Halo-tag labelling in live cells, and highlight enhanced properties in several applications, such as fluorescence activated cell sorting, fluorescence lifetime microscopy, stimulated emission depletion nanoscopy and single-molecule Förster-resonance energy transfer. We finally extend this idea to other dye families and envision deuteration as a generalizable concept to improve existing and to develop new chemical biology probes.
Date Issued
2022-08-07
Date Acceptance
2022-06-02
Citation
Chemical Science, 2022, 13 (29), pp.8605-8617
ISSN
2041-6520
Publisher
The Royal Society of Chemistry
Start Page
8605
End Page
8617
Journal / Book Title
Chemical Science
Volume
13
Issue
29
Copyright Statement
© 2022 The Author(s). Published by the Royal Society of Chemistry Open Access Article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/35974762
PII: d1sc06466e
Subjects
Chemistry
Chemistry, Multidisciplinary
CONFORMATIONAL DYNAMICS
FLUOROGENIC PROBES
GENERAL-METHOD
IMPROVE FLUOROPHORES
LIVE-CELL
Physical Sciences
RATIONAL DESIGN
Science & Technology
SINGLE-MOLECULE
SUPERRESOLUTION MICROSCOPY
Publication Status
Published
Coverage Spatial
England
Date Publish Online
2022-06-28