The role of HLA-DRB1 alleles in pulmonary cystic fibrosis
File(s)
Author(s)
Type
Journal Article
Abstract
Cystic fibrosis (CF) is the most common lethal autosomal recessive disease inwhite Caucasians. It affects many organs including the lung, pancreas, and liver. Whilst CF is a monogenic disease, several studies revealed a complex relationship between genotype and clinical phenotype of diseases.We examined the expression of human leukocyte antigen (HLA) class II alleles among Iranian CF patients with disease-related microbial infection. This study was conducted on 50 hospitalized CF patients (27 males, 23 females aged 15.5±6.5 years), and 50 healthy age-and gender-matched control subjects. 5ml whole blood was harvested and after isolation of genomic DNA, HLA-DRB1 subtypes were determined by single specific primer polymerase chain reaction methods. HLA-DRB1*10 was less frequent and HLA-DRB1*04 and HLA-DRB1*11 wasthe most frequent allele in CF patients, but none reached significance. HLA-DRB1*04 allele was frequently seen among16 CF patients with high serum IgElevels (430.25±219.7 IU/mL) and 27 CF patients that were positive for Pseudomonas aeruginosa colonization. A total of31 CF patients had candida Albicans colonization in whomHLA-DRB1*11 was mostly seen. A total of 3 CF patients had allergic bronchopulmonary aspergillosis and two were diabetic. The DR4 and DR11 serotypes that recognize the HLA-DRB1*04 and HLA-DRB1*11 gene products respectively are not significantly enriched in the Iranian CF population. Further research should be conducted on DR4 and DR11 in CF patients to understand their possible role in infection and IgE expression
Date Issued
2022-04-01
Date Acceptance
2022-04-01
Citation
Iranian Journal Of Allergy, Asthma and Immunology, 2022, 21 (2), pp.189-196
ISSN
1735-1502
Publisher
Tehran University of Medical Sciences
Start Page
189
End Page
196
Journal / Book Title
Iranian Journal Of Allergy, Asthma and Immunology
Volume
21
Issue
2
Copyright Statement
© 2022Asefetal. Published by Tehran University of Medical Sciences.189This work islicensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited
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Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000791830000008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Allergy
Immunology
Cystic fibrosis
HLA-DRB1 chains
Pseudomonas aeruginosa
Staphylococcus aureus
ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS
CANDIDA-ALBICANS
HLA FREQUENCIES
ASSOCIATION
GENES
SUSCEPTIBILITY
EXPRESSION
FUMIGATUS
HAPLOTYPE
IMMUNITY
Publication Status
Published