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X-ray crystallographic studies of RoAb13 bound to PIYDIN, a part of the N-terminal domain of C-C chemokine receptor 5
File(s)
mf5053.pdf (786.17 KB)
Published version
Author(s)
Govada, Lata
Saridakis, Emmanuel
Kassen, Sean C
Bin-Ramzi, Ahmad
Morgan, Rhodri Marc
Type
Journal Article
Abstract
C-C chemokine receptor 5 (CCR5) is a major co-receptor molecule used by HIV-1 to enter cells. This led to the hypothesis that stimulating an antibody response would block HIV with minimal toxicity. Here, X-ray crystallographic studies of the anti-CCR5 antibody RoAb13 together with two peptides were undertaken: one peptide is a 31-residue peptide containing the PIYDIN sequence and the other is the PIDYIN peptide alone, where PIYDIN is part of the N-terminal region of CCR5 previously shown to be important for HIV entry. In the presence of the longer peptide (the complete N-terminal domain), difference electron density was observed at a site within a hypervariable CDR3 binding region. In the presence of the shorter core peptide PIYDIN, difference electron density is again observed at this CDR3 site, confirming consistent binding for both peptides. This may be useful in the design of a new biomimetic to stimulate an antibody response to CCR5 in order to block HIV infection.
Date Issued
2021-07-01
Date Acceptance
2021-05-20
Citation
IUCrJ, 2021, 8 (4), pp.678-683
URI
URL
DOI
ISSN
2052-2525
Publisher
International Union of Crystallography
Start Page
678
End Page
683
Journal / Book Title
IUCrJ
Volume
8
Issue
4
Replaces
10044/1/97888
Copyright Statement
© 2022 The Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
License URL
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000670812900018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
antibodies
ANTIBODIES
CCR5
CCR5 receptor
Chemistry
Chemistry, Multidisciplinary
CRYSTALLIZATION
Crystallography
HIV entry
Materials Science
Materials Science, Multidisciplinary
OIL
Physical Sciences
PIYDIN
REFINEMENT
RoAb13
Science & Technology
structural biology
structure determination
Technology
viruses
X-ray crystallography
Publication Status
Published
Date Publish Online
2021-07-01