Oxidized low-density lipoproteins enhance expression and activity of CD39 and CD73 in the human aortic valve endothelium.
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Accepted version
Author(s)
Type
Journal Article
Abstract
Extracellular nucleotides regulate thrombosis, inflammation and immune response.
Ectonucleoside triphosphate diphosphohydrolase 1 (CD39) and ecto-5'-nucleotidase (CD73)
convert extracellular nucleotides in a sequential order: ATP to ADP, AMP and then to
adenosine. In this study, we aimed to test an effect of oxidized LDL (ox-LDL) on CD39 and
CD73 in endothelial cells.
Human aortic valve endothelial cells were exposed to oxidized low-density
lipoprotein, for 24-48 h. Next, the activity, protein expression and mRNA transcripts level of
CD39 and CD73 were characterised by: an incubation with ATP or AMP followed by HPLC
analysis of media as well as western blots and qPCR. Results are presented as mean ± SEM.
CD73 activity in human valve endothelial cells was increased in presence of ox-LDL
(4.04±0.32 nmol/mg prot./min) as compared to control (2.75±0.21 nmol/mg prot/min). There
was almost no effect of ox-LDL on CD39 activity. A similar effect was observed for mRNA
and protein expression.
In conclusion, we found that ox-LDL modulated CD39 and CD73 activity in the
endothelium, which may contribute to relevant pathologies and featured treatments.
Ectonucleoside triphosphate diphosphohydrolase 1 (CD39) and ecto-5'-nucleotidase (CD73)
convert extracellular nucleotides in a sequential order: ATP to ADP, AMP and then to
adenosine. In this study, we aimed to test an effect of oxidized LDL (ox-LDL) on CD39 and
CD73 in endothelial cells.
Human aortic valve endothelial cells were exposed to oxidized low-density
lipoprotein, for 24-48 h. Next, the activity, protein expression and mRNA transcripts level of
CD39 and CD73 were characterised by: an incubation with ATP or AMP followed by HPLC
analysis of media as well as western blots and qPCR. Results are presented as mean ± SEM.
CD73 activity in human valve endothelial cells was increased in presence of ox-LDL
(4.04±0.32 nmol/mg prot./min) as compared to control (2.75±0.21 nmol/mg prot/min). There
was almost no effect of ox-LDL on CD39 activity. A similar effect was observed for mRNA
and protein expression.
In conclusion, we found that ox-LDL modulated CD39 and CD73 activity in the
endothelium, which may contribute to relevant pathologies and featured treatments.
Date Issued
2016-12-01
Date Acceptance
2016-02-14
Citation
Nucleosides, Nucleotides and Nucleic Acids, 2016, 35 (10-12), pp.713-719
ISSN
1525-7770
Publisher
Taylor & Francis
Start Page
713
End Page
719
Journal / Book Title
Nucleosides, Nucleotides and Nucleic Acids
Volume
35
Issue
10-12
Copyright Statement
© 2016 Taylor & Francis Group, LLC. This is an Author's Accepted Manuscript of an article published in [include the complete citation information for the final version of the article as published in the Nucleosides, Nucleotides and Nucleic Acids (2016), available online at: http://www.tandfonline.com/full/10.1080/15257770.2016.1163377
Sponsor
The Magdi Yacoub Institute
Grant Number
WHCF_P67039
Subjects
CD39
CD73
Ox-LDL
aortic valve
endothelium
Organic Chemistry
0601 Biochemistry And Cell Biology
Publication Status
Published
Date Publish Online
2016-12-01