Void-free 3D bioprinting for in-situ endothelialization and microfluidic perfusion
File(s)
Accepted version
Author(s)
Ouyang, L
Armstrong, J
Chen, Qu
Lin, Yiyang
Stevens, Molly
Type
Journal Article
Abstract
Two major challenges of 3D bioprinting are the retention of structural fidelity and efficient endothelialization for tissue vascularization. We address both of these issues by introducinga versatile3D bioprinting strategy, in which a templating bioink is deposited layer-by-layer alongside a matrix bioink to establish void-free multimaterial structures. After crosslinking the matrix phase, the templating phase issacrificedto create a well-defined 3D network of interconnected tubular channels. This void-free 3D printing (VF-3DP) approachcircumvents the traditional concerns of structural collapse, deformation and oxygen inhibition, moreover, it can be readily used to printmaterials that are widely considered “unprintable”. By pre-loading endothelial cells into the templating bioink, the inner surface of the channels can be efficiently cellularized with a confluent endothelial layer. This in-situ endothelializationmethod can be used to produce endothelium with a far greater uniformity than can be achieved using the conventional post-seeding approach. This VF-3DP approach canalsobe extended beyond tissue fabrication and towards customized hydrogel-based microfluidics and self-supported perfusable hydrogel constructs.
Date Issued
2020-01-03
Date Acceptance
2019-10-19
Citation
Advanced Functional Materials, 2020, 30 (1)
ISSN
1616-301X
Publisher
Wiley
Journal / Book Title
Advanced Functional Materials
Volume
30
Issue
1
Copyright Statement
© 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Sponsor
Engineering & Physical Science Research Council (E
Medical Research Council (MRC)
Wellcome Trust
Medical Research Council
Grant Number
20069192
MR/R015651/1
098411/Z/12/Z
MR/S00551X/1
Subjects
Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Applied
Physics, Condensed Matter
Chemistry
Science & Technology - Other Topics
Materials Science
Physics
bioprinting
endothelialization
hydrogels
microfluidics
printability
TISSUE
HYDROGELS
VASCULARIZATION
NETWORKS
CULTURE
03 Chemical Sciences
09 Engineering
02 Physical Sciences
Materials
Publication Status
Published
Article Number
1908349
Date Publish Online
2019-11-11