Canine distemper virus neutralization activity is low in human serum and it is sensitive to an amino acid substitution in the hemagglutinin protein
File(s)
Author(s)
Type
Journal Article
Abstract
Serum was analyzed from 146 healthy adult volunteers in eastern Africa to evaluate measles virus (MV) and canine distemper virus (CDV) neutralizing antibody (nAb) prevalence and potency. MV plaque reduction neutralization test (PRNT) results indicated that all sera were positive for MV nAbs. Furthermore, the 50% neutralizing dose (ND50) for the majority of sera corresponded to antibody titers induced by MV vaccination. CDV nAbs titers were low and generally were detected in sera with high MV nAb titers. A mutant CDV was generated that was less sensitive to neutralization by human serum. The mutant virus genome had 10 nucleotide substitutions, which coded for single amino acid substitutions in the fusion (F) and hemagglutinin (H) glycoproteins and two substitutions in the large polymerase (L) protein. The H substitution occurred in a conserved region involved in receptor interactions among morbilliviruses, implying that this region is a target for cross-reactive neutralizing antibodies.
Date Issued
2015-04-14
Date Acceptance
2015-03-17
Citation
Virology, 2015, 482, pp.218-224
ISSN
1096-0341
Publisher
Elsevier
Start Page
218
End Page
224
Journal / Book Title
Virology
Volume
482
Copyright Statement
© 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Identifier
http://www.ncbi.nlm.nih.gov/pubmed/25880113
Subjects
Canine distemper virus Cross-neutralization Hemagglutinin Measles virus Neutralizing antibody Plaque reduction neutralization test Pre-existing immunity
Notes
Zhang, Xinsheng Wallace, Olivia L Domi, Arban Wright, Kevin J Driscoll, Jonathan Anzala, Omu Sanders, Eduard J Kamali, Anatoli Karita, Etienne Allen, Susan Fast, Pat Gilmour, Jill Price, Matt A Parks, Christopher L ENG 2015/04/17 06:00 Virology. 2015 Apr 13;482:218-224. doi: 10.1016/j.virol.2015.03.035. Serum was analyzed from 146 healthy adult volunteers in eastern Africa to evaluate measles virus (MV) and canine distemper virus (CDV) neutralizing antibody (nAb) prevalence and potency. MV plaque reduction neutralization test (PRNT) results indicated that all sera were positive for MV nAbs. Furthermore, the 50% neutralizing dose (ND50) for the majority of sera corresponded to antibody titers induced by MV vaccination. CDV nAbs titers were low and generally were detected in sera with high MV nAb titers. A mutant CDV was generated that was less sensitive to neutralization by human serum. The mutant virus genome had 10 nucleotide substitutions, which coded for single amino acid substitutions in the fusion (F) and hemagglutinin (H) glycoproteins and two substitutions in the large polymerase (L) protein. The H substitution occurred in a conserved region involved in receptor interactions among morbilliviruses, implying that this region is a target for cross-reactive neutralizing antibodies.
Publication Status
Published