LOCUS       pYC1.8_Hsp70P[NL        8036 bp    DNA     circular     15-Sep-2011
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     misc_feature    2556..2559
                     /label="Imported from vectorNTI*10"
                     /note="misc_feature GAP ~4bp sequence/length unknown"
     misc_feature    2560..4433
                     /label="Imported from vectorNTI*12"
                     /note="misc_feature v[+]t1.8 selectable marker"
     misc_recomb     7437..8023
                     /label="Imported from vectorNTI*11"
                     /note="misc_recomb p5' (P Element)"
     misc_feature    4444..4676
                     /label="Imported from vectorNTI*14"
                     /note="misc_feature p3' (P Element)"
     misc_feature    4434..4443
                     /label="Imported from vectorNTI*13"
                     /note="misc_feature Gap ~10bp uncertain length"
     old_sequence    5179..7208
                     /label="Imported from vectorNTI*16"
                     /note="old_sequence pUC8 region"
     misc_feature    4677..5178
                     /label="Imported from vectorNTI*15"
                     /note="misc_feature w[+]car6.1 3'"
     source          7437..8023
                     /label="Imported from vectorNTI*2"
                     /note="source Source_1"
     CDS             5583..6440
                     /label="Imported from vectorNTI*18"
                     /note="CDS AmpR"
     source          2556..2559
                     /label="Imported from vectorNTI*3"
                     /note="source Source_2"
     misc_feature    7209..7436
                     /label="Imported from vectorNTI*17"
                     /note="misc_feature w[+]car6.1 5'"
     terminator      7..274
                     /label="Imported from vectorNTI*20"
                     /note="terminator Hsp70T"
     promoter        1106..2535
                     /label="Imported from vectorNTI*19"
                     /note="promoter Hsp70 Promoter"
     source          7209..7436
                     /label="Imported from vectorNTI*9"
                     /note="source Source_8"
     source          5179..7208
                     /label="Imported from vectorNTI*8"
                     /note="source Source_7"
     source          4434..4443
                     /label="Imported from vectorNTI*5"
                     /note="source Source_4"
     source          2560..4433
                     /label="Imported from vectorNTI*4"
                     /note="source Source_3"
     source          4677..5178
                     /label="Imported from vectorNTI*7"
                     /note="source Source_6"
     source          4444..4676
                     /label="Imported from vectorNTI*6"
                     /note="source Source_5"
     CDS             complement(278..1105)
                     /label="Imported from vectorNTI"
                     /note="CDS NLS-HA-I-SceI"
ORIGIN
        1 GGCCGCGAAT TCGAGCTCGC CCGGGGATCT AAACGAGTTT TTAAGCAAAC TCACTCCCTG 
       61 ACAATAAAAA CGCAATTATC TTTAAATAAA TATATTCTAT TTATTAACCA AGTAGCTAAT 
      121 ATAGCTAAAA TCAATTTGTT GCTAACTTAA AACTAACATA ATTATGTATT AGTTATTTAC 
      181 ATTCTTTTGG TTGGTTACAA CCAAATAAAT AATATCACTT TTAAAAACTT AAGCCAGGAA 
      241 CTGAATAATT TTCTATTTGG CTTTAGTCGA CTTATTATTT CAGGAAAGTT TCGGAGGAGA 
      301 TAGTGTTCGG CAGTTTGTAC ATCATCTGCG GGATCAGGTA CGGTTTGATC AGGTTGTAGA 
      361 AGATCAGGTA AGACATAGAA TCGATGTAGA TGATCGGTTT GTTTTTGTTG ATTTTTACGT 
      421 AACAGTTCAG TTGGAATTTG TTACGCAGAC CCTTAACCAG GTATTCTACT TCTTCGAAAG 
      481 TGAAAGACTG GGTGTTCAGT ACGATCGATT TGTTGGTAGA GTTTTTGTTG TAATCCCATT 
      541 TACCACCATC ATCCATGAAC CAGTATGCCA GAGACATCGG GGTCAGGTAG TTTTCAACCA 
      601 GGTTGTTCGG GATGGTTTTT TTGTTGTTAA CGATGAACAG GTTAGCCAGT TTGTTGAAAG 
      661 CTTGGTGTTT GAAAGTCTGG GCGCCCCAGG TGATTACCAG GTTACCCAGG TGGTTAACAC 
      721 GTTCTTTTTT GTGCGGCGGG GACAGTACCC ACTGATCGTA CAGCAGACAT ACGTGGTCCA 
      781 TGTATGCTTT GTTTTTCCAC TCGAACTGCA TACAGTAGGT TTTACCTTCA TCACGAGAAC 
      841 GGATGTAAGC ATCACCCAGG ATCAGACCGA TACCTGCTTC GAACTGTTCG ATGTTCAGTT 
      901 CGATCAGCTG GGATTTGTAT TCTTTCAGCA GTTTAGAGTT CGGACCCAGG TTCATTACCT 
      961 GGTTTTTTTT GATGTTTTTC ATACCCGCAT AGTCAGGAAC ATCGTATGGG TACATAAATC 
     1021 GTGGGTCTTC TACTTTTCGT TTTTTTTTTG GTGGTGCTGC ATGCTGCGCA TCGGCCGTAG 
     1081 CTTCGTCGTC TGATGATGAT CCCATAGTGG CGGCGGATCT GAATTGATCC TGTGTGTGAG 
     1141 TTCTTCTTTC TCGGTAACTT GTTGAAAGTA TTCAGAGTTC TCTTCTTGTA TTCAATAATT 
     1201 ACTTCTTGGC AGATTTCAGT AGTTGCAGTT GATTTACTTG GTTGCTGGTT ACTTTTAATT 
     1261 GATTCACTTT AACTTGCACT TTACTGCAGA TTGTTTAGCT TGTTCAGCTG CGCTTGTTTA 
     1321 TTTGCTTAGC TTTCGCTTAG CGACGTGTTC ACTTTGCTTG TTTGAATTGA ATTGTCGCTC 
     1381 CGTAGACGAA GCGCCTCTAT TTATACTCCG GCGCTCTTTT CGCGAACATT CGAGGCGCGC 
     1441 TCTCTCGAAC CAACGAGAGC AGTATGCCGT TTACTGTGTG ACAGAGTGAG AGAGCATTAG 
     1501 TGCAGAGAGG GAGAGACCCA AAAAGAAAAG AGAGAATAAC GAATAACGGC CAGAGAAATT 
     1561 TCTCGAGTTT TCTTTCTGCC AAACAAATGA CCTACCACAA TAACCAGTTT GTTTTGGGAT 
     1621 TCTAGAATAT TCGCTTTATT TTGGAAATTT CTTTATAAAT ACGGCTGCTT AAATAACTTA 
     1681 TGTTAAAGAT AATCGAAGCG CTTTGTTATG CGTATTCGCA AAAAAAATAC TGCGCTTCTA 
     1741 AACCAATAAA TGTCTGATCG GCATATGTAT TAAAGATTAT TTTGTACTTT TAGTTAATGA 
     1801 AATCAACTAT TAACGGACTT TCTTCTGTTT TAATATACGA TAAAATTAAT TTAAGATTCT 
     1861 GAAAAATGTA TTAGAATTTA GTTGCGACTT ATTTTACATA TTTACATACA TATGTACATA 
     1921 TGAGGAAAGC CAAGACTATT ACATACAGTT TATCAAGTTT ACAAACTGTG AAATATGTCG 
     1981 AATTTTTGAC TTTAAAGGTA AAAATTAAGG GTTATTTGCT TAATTAAACG GAATTTTTTT 
     2041 TTTTAATATA ATTAAACAAT ATTTATTTTA CTTATAAATA AAAAAAAAAT TAAAAATATT 
     2101 AGATATACAG TTTGTCAAGA AACTGTTTAC ACACTGTGAA ATAAGTTGAA TTTTTGACTT 
     2161 TAAAGCTAAA AATTAAGGGT TTTTTGCTTA ATTAAACGCA ATTTTTTTAT AAAATATAAT 
     2221 TAAACAATAT TTATTTTACT TATAAATCAA AAAATAAATT AAAAATATTA ATTATACAAG 
     2281 AAAATAAACC ACAAATTCCA AGTTTACACA CTTTTGAGAC TGTCAAGAAA CTCTTTACAC 
     2341 AAAACACTAA ATCGAAGTTT AATCCTAAAA TTTTAAATTA TTTAATTAAA ATTTTAAAAG 
     2401 TTTTAGTACG TAGTATGTTC CTCATTGACT TTGTCTACGA GTTGGAGACT GTTTTTATGG 
     2461 AATGGTTACG TTTTTGTAGG TCATATTCAA GATCCTCTAG AGTCGATCGA CCTGCAGCCC 
     2521 AAGCTTATCG ATACCGTCGA CCTGCAGCCA AGCTTTATTT ATTTTGTTAT GTTATATGTA 
     2581 TTATATGTCA GACATAAAGA AAAGGAACAC ATCAAATGTG ATAACAAAGA CTAAACAAGT 
     2641 AATTTTATTA CACCAAAACG ACAAAACAGT AGGCAGAACA AACAACGCAT AGCCAAACAT 
     2701 TGACCAATTG GATACCCTGC CGATTGTCAG ACACTTTTGT TGATCAGTTT CTTGCGAATG 
     2761 GTCTCGTCCA GCGGTGGAAT CGCCTCGCGG GGAATCAGAA AAGTGGACAG ATTGAACAGA 
     2821 TCCAGAAACA CCTTGTACCG ATCACTGAAA CCAAAAAAAA ACAAAGGGAG AACAGTTTGA 
     2881 GTTCATTGAT CCCCGATATA ATCACATCTG CGATGATCAC CTGAGAGTGG AGCGCAGATA 
     2941 TTGATATCCA GACGAGCCAC CAGTGCCCAA CTGTTGGGAT CCAATCATGC GTTGCACCAT 
     3001 GATCACGTGA TTGTCTGCGG CGGGAATAGA AAGTATTTGG TTAGGAAAAC CAGTCTTAAA 
     3061 CATAAGATAT ATTTATAAAA GAGTATCAAA GAATGCAATA CTTACATCTC CACTTGGTTA 
     3121 TTAACGAGTC GATGTCCATG AGCAGGGTGA GCAACTGGTG TGGTTGGCTG AACCTGGGTT 
     3181 CATCCCTATA GAAGGTGATC ATGATGGCTC CCTGAAGGGC ACGATGGCTA AACCGGCGAT 
     3241 CCCCACGACG CACCAGTGCA TCGTGCACTG CCGGATCAAA GATGGAGCGA TACACCTCGC 
     3301 GTCGCTTCTC AATGTCCATG AGGCGGTAGT TTTTCGCCTT CTCCACGGGC TCCTCCATGG 
     3361 CGCTCTGTAC CTGCGCCTCC AGGAATCGAT CGACGCTCTC CTGAAACTTG GCCCAGAAGT 
     3421 TGAAGCCACT CTCCTCCAGT CCGGGCGTCC TCTCCAGCCA TCGCTGCACT AGCTCCAGTA 
     3481 GCGAGGGATC TTTCTCCGAG TTGCGAATCG AATTCCGCGC CTCCTCGTCG CTAAAGACAT 
     3541 CCGAGTACTT CTGGTTGTAT CTCACCCGCT GCTCTGTCAG AACTCCCAGC TTGTTCTCGA 
     3601 TCAAACGGAA CTGCAGCGAC TGAAAACCAG ATGCGGGTGC CAGGTACTTG CGGAAGTCCA 
     3661 TGAAGTCTAG CGGGGTCATG GTCTCCAGAA TGGGCACTTG GTCCACCAGG AGCTGTACAA 
     3721 AGGAAGTTAT AAACGGATTT TGGTAAGAGA TTCAGAAAGC ACTCACTTTT AGAATCAGAA 
     3781 CCACTCGGTT CAGTCGCTTG ACAATCTCCA GCGTCTTGGT TTCATCGATG ACCTCTGCAT 
     3841 CCAACATGTC TCGTATGGAG TCGAACTCAA AGATGATCTG CTTGAACCAA AGCTCGTAGG 
     3901 CTGTGGCGAA GGTACTTAAA TGCCATTGAG TGTTGTCATC AAAGTTGTAA ACCTACTCAC 
     3961 CCTGGTGCGT GATGATGAAC AGATGCTCAT CGTGCACGGG TCGCTTGTCC TCCTCGGACA 
     4021 GCATACACTG GGCATCCAGC AGTTTGTCCA GCATCAGATA CTCTCCATAG ATTTTGCCCA 
     4081 CTTCCGTGGT TAATGGCACC GCCGAATCAT CGTGATCGTT TCTGTATGGG TTTGAATTGA 
     4141 ATCGCAGAAC TGAAGATCGA TTGGCATTCC TGGACAGCAC GTGCTGGTGC TCACCCGTTT 
     4201 CCTGCATAGG GACAGCTCAT GGTGCACAGC TCAGATCAGA TCGTGACTCC TCGAGCGGCG 
     4261 GATGCTGGCG AACTGATCTC CGCCAGCGGA CCGGAGATGA GACCCCAGCG AACCGATAAC 
     4321 AGAGCGAGAG AGCTCCAGTT CCGACTGATT GCACAGTCGG TGATCTGGGC GATGGGCACT 
     4381 GCCAGATAGG CTGGGAATTA TCAATCACTT GAGGTGAAAG TGCGGCGCAC ACAAATCAAG 
     4441 CTTTGCGTAC TCGCAAATTA TTAAAAATAA AACTTTAAAA ATAATTTCGT CTAATTAATA 
     4501 TTATGAGTTA ATTCAAACCC CACGGACATG CTAAGGGTTA ATCAACAATC ATATCGCTGT 
     4561 CTCACTCAGA CTCAATACGA CACTCAGAAT ACTATTCCTT TCACTCGCAC TTATTGCAAG 
     4621 CATACGTTAA GTGGATGTCT CTTGCCGACG GGACCACCTT ATGTTATTTC ATCATGGTCT 
     4681 GGCCATTCTC ATCGTGAGCT TCCGGGTGCT CGCATATCTG GCTCTAAGAC TTCGGGCCCG 
     4741 ACGCAAGGAG TAGCCGACAT ATATCCGAAA TAACTGCTTG TTTTTTTTTT TACCATTATT 
     4801 ACCATCGTGT TTACTGTTTA TTGCCCCCTC AAAAAGCTAA TGTAATTATA TTTGTGCCAA 
     4861 TAAAAACAAG ATATGACCTA TAGAATACAA GTATTTCCCC TTCGAACATC CCCACAAGTA 
     4921 GACTTTGGAT TTGTCTTCTA ACCAAAAGAC TTACACACCT GCATACCTTA CATCAAAAAC 
     4981 TCGTTTATCG CTACATAAAA CACCGGGATA TATTTTTTAT ATACATACTT TTCAAATCGC 
     5041 GCGCCCTCTT CATAATTCAC CTCCACCACA CCACGTTTCG TAGTTGCTCT TTCGCTGTCT 
     5101 CCCACCCGCT CTCCGCAACA CATTCACCTT TTGTTCGACG ACCTTGGAGC GACTGTCGTT 
     5161 AGTTCCGCGC GATTCGGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT 
     5221 GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT 
     5281 GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT 
     5341 GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA 
     5401 TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA 
     5461 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA 
     5521 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA 
     5581 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC 
     5641 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG 
     5701 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC 
     5761 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT 
     5821 CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT 
     5881 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT 
     5941 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA 
     6001 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC 
     6061 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA 
     6121 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG 
     6181 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC 
     6241 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT 
     6301 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT 
     6361 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG 
     6421 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG 
     6481 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA 
     6541 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA 
     6601 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA 
     6661 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA 
     6721 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT 
     6781 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT 
     6841 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT 
     6901 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT 
     6961 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA 
     7021 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG 
     7081 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC 
     7141 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA 
     7201 AAAACGCCTT CTTCTTGAAC TCGGGCTCGG TGCCAGTATA CCTCAAATGG TTGTCGTACC 
     7261 TCTCATGGTT CCGTTACGCC AACGAGGGTC TGCTGATTAA CCAATGGGCG GACGTGGAGC 
     7321 CGGGCGAAAT TAGCTGCACA TCGTCGAACA CCACGTGCCC CAGTTCGGGC AAGGTCATCC 
     7381 TGGAGACGCT TAACTTCTCC GCCGCCGATC TGCCGCTGGA CTACGTGGGT CTGGCCCATG 
     7441 ATGAAATAAC ATAAGGTGGT CCCGTCGATA GCCGAAGCTT ACCGAAGTAT ACACTTAAAT 
     7501 TCAGTGCACG TTTGCTTGTT GAGAGGAAAG GTTGTGTGCG GACGAATTTT TTTTTGAAAA 
     7561 CATTAACCCT TACGTGGAAT AAAAAAAAAT GAAATATTGC AAATTTTGCT GCAAAGCTGT 
     7621 GACTGGAGTA AAATTAATTC ACGTGCCGAA GTGTGCTATT AAGAGAAAAT TGTGGGAGCA 
     7681 GAGCCTTGGG TGCAGCCTTG GTGAAAACTC CCAAATTTGT GATACCCACT TTAATGATTC 
     7741 GCAGTGGAAG GCTGCACCTG CAAAAGGTCA GACATTTAAA AGGAGGCGAC TCAACGCAGA 
     7801 TGCCGTACCT AGTAAAGTGA TAGAGCCTGA ACCAGAAAAG ATAAAAGAAG GCTATACCAG 
     7861 TGGGAGTACA CAAACAGAGT AAGTTTGAAT AGTAAAAAAA ATCATTTATG TAAACAATAA 
     7921 CGTGACTGTG CGTTAGGTCC TGTTCATTGT TTAATGAAAA TAAGAGCTTG AGGGAAAAAA 
     7981 TTCGTACTTT GGAGTACGAA ATGCGTCGTT TAGAGCAGCA GCCGAATTCC CGTTGC
//