LOCUS       pENTRR4-Vas2(I-S        6847 bp    DNA     circular     10-Sep-2011
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     misc_feature    3063..3893
                     /label="I-SceI CDS "
     misc_feature    760..3050
                     /label="Imported from vectorNTI*5"
                     /note="misc_feature 5'Vas_Int"
     misc_feature    537..552
                     /label="Imported from vectorNTI*4"
                     /note="misc_feature M13F"
     misc_feature    3903..4903
                     /label="Vas2 3' UTR"
     misc_feature    4909..5033
                     /label="Imported from vectorNTI*2"
                     /note="misc_feature attR3"
     misc_feature    603..727
                     /label="Imported from vectorNTI"
                     /note="misc_feature attR4"
     misc_feature    5152..5170
                     /label="Imported from vectorNTI*3"
                     /note="misc_feature pDONR-RP"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCT ACAGGTCACT AATACCATCT AAGTAGTTGA 
      601 TTCATAGTGA CTGGATATGT TGTGTTTTAC AGTATTATGT AGTCTGTTTT TTATGCAAAA 
      661 TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC TCGTTCAACT TTTCTATACA 
      721 AAGTTGGTAC CGGGCCCCCC GCTAGCGTCG ACGGTATCGA TGTAGAACGC GAGCAAATTC 
      781 TTTTCCTTCC ATGACAGCAG CAGCTACAGT GGGAAGCCGA ACGTCAGACG TGTTTGACAT 
      841 GCCGAACTGG GCGGGAAAAT TACAGCGTGC GCTTTGTTTT CAAGCAAATC ACAACTCGCT 
      901 GCAAACAAAA CCGTTGAGAA ATTGATTGTT TTATAATTTG TATTGTATTT TATTTGTTAT 
      961 AATAAACTAA AAAGACATAC TTTTTGCATA TTTTATACAT AAAAACATAC ATGCAGCATT 
     1021 ATAAAACACA TATAAACCCT CCCTGTAGAG TCCCGTATCG AAATCTTCCA TCCTAGTTGC 
     1081 ACAGTACGAC GGACGAGTAG GCCGTGTCCG TGCAAATTCC AGCTTTTAGC AGTCTTTTGC 
     1141 TCGGAGCACT CGCGGCGAGT CGGAGGTTTC TGCTGAGGTG CTTAGCGCTA AATTAGCCAA 
     1201 TTGCTTTTGC AAGTGAAATA ACCAGCCGAA TAGTACTTCA AAACTCAGGT AAGTGAACTA 
     1261 GTTTTATAGA ACAAATGTTT GTTTGTTAGA AGTTAGTGAA GTGTTTGTGA AAAAAATCTC 
     1321 TCATTTCGGC AAAACTAACG TAACTGATTT CAAATTGAAT TATTGTTTTG TGATGTTATA 
     1381 TTATTTCATC CAGTTGATTA GTATTTTCTT AGTTATGTTC AAAATACAGT TAAATTAAAT 
     1441 TTCATTTCAT TTACTCATAA AATAATCTCT TGGCTTATTT AATTTTTCTC GAATTCGCTT 
     1501 GTATTGTTCA GTAGCACGCG CCATTCGCCC TTTGTTTCAT TTTGTACCTG CTCCCACTAA 
     1561 CACACTGGCA GTGCGAAACA AAAGCCTTCG CACGCGTTGC TGGTATTAGA GTGTGTGCGT 
     1621 GTGTGTGTTG AGCGCTCTGT CAAAATCGGC TGTTGCCGCC GGTACCGAAA TTGCCTGTTC 
     1681 GCACGCTGTT CGTAAACATT CCGTGGTGTG TATCGTGTGT TGTGCATGTT GCGCGCCTCC 
     1741 CCCCTTTTGA TAGCAGGCTG CCGTGGCTGC CGTGGTGTGT GGCGCAGTTG AGTTTTTGGA 
     1801 TTAATTTTCT AAGGAAATGG CACGAGAAGA GCGGTGGCAG TGTGTTGGTT TGCTCTGTCC 
     1861 CTTCCTTTCT GTGTGAAGTG TTCTTACAGC ACAGCACGTA TCCACCACCG CACACAGAGC 
     1921 AGGCAAGGAA GTGGAAGTGA ACAAGTGTGC TGCGCATGCA TGTGTGTGGG GGGCATTTTA 
     1981 GCTGAGATCG TCGTTATTTG AGAAGCGGTA TAGGGGCCAG TCGGTGTCGA CGTACGGAAG 
     2041 CGGTTTAGTT TTAATCCAAG CGTATCCCGT CGTGGAGTGG TTGTGTGGCT CTGTGTGCTC 
     2101 TCATATCAGT TCCAGAGTGA GGTTAGTAGA ATCACAGTCC TTGGCCTTTT TCGTTACAAG 
     2161 ATATCCAGAA GGATGGCGTT ATTTCCACAG CTTACCATGG TGCTCTTGTT TGCTCGAATC 
     2221 AGGGGAGAAA AACAGTTTCG TGTTTCATGA ACCGCAGTTG GCACTGGAGC GGATTCAAAA 
     2281 GTCTTCGATA TGCAATAGAT AAGAGAGTCG TTGGGGCATA GTTGGGAAGC CTTTCCGAGA 
     2341 TGTGGAGTTT CCGAGAGGAG AAATGGTGCT TTCGTGCACG TTCCGGGACA GCGGGCCCCG 
     2401 CGAAGAGCAT CTCGTTGTCG TTCATCCGGC AATAATTGAT GCGAAAAGCG CGCGCGCCAC 
     2461 TGGCTTAGCG CAGTGTACAC AGTGATATTC ACCTACACAC ACAGAGGCAC ACGCCTTCAC 
     2521 ACGCGCGCGT GCTTCAAAGG CTACTTCGGT GGCGGTGTGT GAGGTCGCTT GCAATGGACA 
     2581 ATGAAAATTT CGCTGGAAAA TACCATCGTC TCTTTAGGTT GCAATGGGTG CGGGTAGAGC 
     2641 GGTGGTCGTC GATATTGGTG GTGTAGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
     2701 TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
     2761 TGCAACGGCA ATTATTTTTT GTAATATTTC GACCATCTTT CTTTCTCTCT CTCCACGTGC 
     2821 TGCTGCTGTT GCTGCTGCTG CTGCATTGCA TGTTCCACTA TTCCTCTCGG TTTGTGCCTG 
     2881 CGGACGCCAT TGCTAGTCGA AAGAGAGTCG CCGTTAGTCG CGCTTCGAGC AACGGACACG 
     2941 TTTTTTGGTT GAAACCAACA GCTTTTTTCA TCTTCGGGAG ACACACAGAT CTCGAATCGT 
     3001 ACATTCCCAT AAGGAGAATT GTCATCTTCC GGTGAATAAA GAAAGGAAAC GGATCCGGAA 
     3061 ACATGGGATC ATCATCAGAC GACGAAGCTA CGGCCGATGC GCAGCATGCA GCACCACCAA 
     3121 AAAAAAAACG AAAAGTAGAA GACCCACGAT TTATGTACCC ATACGATGTT CCTGACTATG 
     3181 CGGGTATGAA AAACATCAAA AAAAACCAGG TAATGAACCT GGGTCCGAAC TCTAAACTGC 
     3241 TGAAAGAATA CAAATCCCAG CTGATCGAAC TGAACATCGA ACAGTTCGAA GCAGGTATCG 
     3301 GTCTGATCCT GGGTGATGCT TACATCCGTT CTCGTGATGA AGGTAAAACC TACTGTATGC 
     3361 AGTTCGAGTG GAAAAACAAA GCATACATGG ACCACGTATG TCTGCTGTAC GATCAGTGGG 
     3421 TACTGTCCCC GCCGCACAAA AAAGAACGTG TTAACCACCT GGGTAACCTG GTAATCACCT 
     3481 GGGGCGCCCA GACTTTCAAA CACCAAGCTT TCAACAAACT GGCTAACCTG TTCATCGTTA 
     3541 ACAACAAAAA AACCATCCCG AACAACCTGG TTGAAAACTA CCTGACCCCG ATGTCTCTGG 
     3601 CATACTGGTT CATGGATGAT GGTGGTAAAT GGGATTACAA CAAAAACTCT ACCAACAAAT 
     3661 CGATCGTACT GAACACCCAG TCTTTCACTT TCGAAGAAGT AGAATACCTG GTTAAGGGTC 
     3721 TGCGTAACAA ATTCCAACTG AACTGTTACG TAAAAATCAA CAAAAACAAA CCGATCATCT 
     3781 ACATCGATTC TATGTCTTAC CTGATCTTCT ACAACCTGAT CAAACCGTAC CTGATCCCGC 
     3841 AGATGATGTA CAAACTGCCG AACACTATCT CCTCCGAAAC TTTCCTGAAA TAAGAGCTCG 
     3901 AGCTTGGGGT GGGGTTGTTA TGTGTTGCGA ACGAGAGTGG ATCTCTCTCG ACATTTCCTT 
     3961 ATTTTTTTTT TCTATTGTTA ACACTTACAA CGAAACTTCG GAAGAGAAGT TTCCTCATTC 
     4021 GAAACGAGGA GTCAAACTCC TTTCCTTGCT TTTGTGACAT GCATGATTAT TCTTTCATTT 
     4081 ACTGACGTAA CGATGTAAAA CACACAGAAG AAGCATGACA CACAGCAAAG AATTGTTCGT 
     4141 TAATAAAACG TTGATGAAAA CTTTGAAAAC ATAAGAACTT GCACTTTTAT TCTATAATTC 
     4201 GTGAAAGCTT GCACCGATTG TCTTTCATTA TTCAATGTAA TGTACTGAAA GGTGATTTTT 
     4261 CGCACTTGTA TACTCTAGAA CTGAAGTATT CTAACAATAC GTCACCTTTA GGTCCATTCC 
     4321 AGGATAAAAT ACACAAGTGA AGGTAGTTGT ACAAAGTACT TAGACTAACC CAGGTTTCAA 
     4381 AAAAGATAAC GACGAATTGA GCGTAACTGC ACAAAGCCCG TTCATTGTCA ACATATCGTT 
     4441 CAGGGGTCTT CAAACTTTAT TATCAGCCCG CGGGCCGCAA CAACGTTGCT TAGAGCACCA 
     4501 TCTTGATGTA TCCTTTATCT AGTGGCTTAT CGTTTATGTC TCGTTTGGCT AAGAAAATAC 
     4561 TAATAACATA TTCAATTTAC GGAGCGGTAC CATGGTACAA ACGTCATACT GCTAGACTTG 
     4621 ACAACATGCC CATCGAGGGT TCTTACCTCA AATGGACCGA TCCCTTGTAA CTGGCGGCGT 
     4681 AAACTCAGTA AATTCCAAAA GCCAGTATAG GCCACCATGA CCGTCTAACT ATTCCAAAAA 
     4741 GGAGAATATT TGAAATTTCT ATATCTATAG TATTATTACT ATATCTATAT GTTTCTTTAG 
     4801 TACAATATTA GAGCTTGATT ATTTTCCTGA AAATTGTAAA ATATGCATGT TAGATGGTTG 
     4861 ATACATCGTT TTAAAAAAAA CTGCTGTTAA TGGCCACATT TTCTCGAGAC AACTTTGTAT 
     4921 AATAAAGTTG AACGAGAAAC GTAAAATGAT ATAAATATCA ATATATTAAA TTAGATTTTG 
     4981 CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA TCCAGTCACT ATGAATCAAC 
     5041 TACTTAGATG GTATTAGTGA CCTGTACTGC AGGGCGGCCG CGATATCCCC TATAGTGAGT 
     5101 CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT 
     5161 GTTACATTGC ACAAGATAAA AATATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA 
     5221 ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA 
     5281 ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT 
     5341 CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC 
     5401 ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA 
     5461 CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT 
     5521 TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT 
     5581 CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG 
     5641 TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA 
     5701 TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG 
     5761 AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC 
     5821 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG 
     5881 ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC 
     5941 TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC 
     6001 CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG 
     6061 TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT 
     6121 GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA 
     6181 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA 
     6241 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC 
     6301 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT 
     6361 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC 
     6421 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC 
     6481 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA 
     6541 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG 
     6601 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG 
     6661 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT 
     6721 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT 
     6781 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC 
     6841 ACATGTT
//