LOCUS       pENTRL1-FRT-PAC-        4632 bp    DNA     circular     15-Sep-2011
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     misc_feature    1327..1484
                     /label="Imported from vectorNTI*8"
                     /note="misc_feature SV40term"
     misc_feature    2893..2911
                     /label="Imported from vectorNTI*5"
                     /note="misc_feature M13R"
     misc_feature    537..553
                     /label="Imported from vectorNTI*4"
                     /note="misc_feature M13F"
     misc_feature    675..708
                     /label="Imported from vectorNTI*6"
                     /note="misc_feature FRT"
     misc_feature    570..665
                     /label="Imported from vectorNTI*2"
                     /note="misc_feature attL1"
     misc_feature    2937..2955
                     /label="Imported from vectorNTI"
                     /note="misc_feature DONR-RP"
     misc_feature    2747..2842
                     /label="Imported from vectorNTI*3"
                     /note="misc_feature attL4"
     misc_feature    715..1326
                     /label="PAC"
     misc_feature    1738..2454
                     /label="Reporter enhanced_green_fluorescent_protein_(EGFP)_ORF"
                     /note="EGFP"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 
      661 AGCAGGCTGG TACCGAAGTT CCTATTCTCT AGAAAGTATA GGAACTTCGT CGACAGGAGG 
      721 GCCACCATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG 
      781 GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT 
      841 CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG 
      901 CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG 
      961 CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG 
     1021 AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC 
     1081 AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT 
     1141 CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC 
     1201 TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC 
     1261 ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT 
     1321 GCCTGACTCG ACCTCGAAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     1381 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     1441 AACTCATCAA TGTATCTTAT CATGTCTGGA TCATGTCGAG GGGGATCGAT AAGCTTGATA 
     1501 TCGAATTCGC CCGGATCTAA TTCAATTAGA GACTAATTCA ATTAGAGCTA ATTCAATTAG 
     1561 GATCCAAGCT TATCGATTTC GAACCCTCGA CCGCCGGAGT ATAAATAGAG GCGCTTCGTC 
     1621 TACGGAGCGA CAATTCAATT CAAACAAGCA AAGTGAACAC GTCGCTAAGC GAAAGCTAAG 
     1681 CAAATAAACA AGCGCAGCTG AACAAGCTAA ACAATCGGCT CGAGACCGGT CGCCACCATG 
     1741 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC 
     1801 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC 
     1861 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC 
     1921 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG 
     1981 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC 
     2041 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG 
     2101 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG 
     2161 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC 
     2221 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC 
     2281 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 
     2341 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG 
     2401 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC 
     2461 GGCCGCGACT CTAGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA 
     2521 AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA 
     2581 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 
     2641 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 
     2701 AGCTACTAGT TCTAGAGCGG CCGCCACCGC GTGGAGCTCG AGCACCCAAC TTTTCTATAC 
     2761 AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC 
     2821 AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGCAGGGC GGCCGCGATA TCCCCTATAG 
     2881 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT 
     2941 CTGATGTTAC ATTGCACAAG ATAAAAATAT ATCATCATGA ACAATAAAAC TGTCTGCTTA 
     3001 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG 
     3061 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG 
     3121 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT 
     3181 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG 
     3241 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC 
     3301 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC 
     3361 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT 
     3421 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC 
     3481 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC 
     3541 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT 
     3601 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG 
     3661 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA 
     3721 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA 
     3781 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA 
     3841 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG 
     3901 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT 
     3961 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 
     4021 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 
     4081 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA 
     4141 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 
     4201 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 
     4261 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 
     4321 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA 
     4381 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 
     4441 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 
     4501 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 
     4561 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 
     4621 TGCTCACATG TT
//